protocols

With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions.В In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals.В For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to t...

Univ. of Washington, Seattle. Guide for using proteomics in research. Aid to better understanding of how cells work and in new possibilities for drug discovery. For researchers.

Expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the co-culture of different cell types; and the detection and treatment of contamination.

Porton Down, Salisbury, UK. Reveals new understanding of molecular mechanisms and applies this new understanding to the development of vaccines. Includes reproducible techniques for researchers. Discusses genetic manipulation of viruses and bacteria for producing live vaccines. For researchers. DNLM: Vaccines.

All you need to know about deploying VoIP protocols in one comprehensive and highly practical reference - Now updated with coverage on SIP and the IMS infrastructure This book provides a comprehensive and practical overview of the technology behind Internet Telephony (IP), providing essential information to Network Engineers, Designers, and Managers who need to understand the protocols. Furthermore, the author explores the issues involved in the migration of existing telephony infrastructure to an IP - based real time communication service. Assuming a working knowledge of IP and networking, it addresses the technical aspects of real-time applications over IP. Drawing on his extensive research and practical development experience in VoIP from its earliest stages, the author provides an accessible reference to all the relevant standards and cutting-edge techniques in a single resource. Key Features: * Updated with a chapter on SIP and the IMS infrastructure * Covers ALL the major VoIP protocols – SIP, H323 and MGCP * Includes a large section on practical deployment issues gleaned from the authors’ own experience * Chapter on the rationale for IP telephony and description of the technical and business drivers for transitioning to all IP networks This book will be a valuable guide for professional network engineers, designers and managers, decision makers and project managers overseeing VoIP implementations, market analysts, and consultants. Advanced undergraduate and graduate students undertaking data/voice/multimedia communications courses will also find this book of interest. Olivier Hersent founded NetCentrex, a leading provider of VoIP infrastructure for service providers, then became CTO of Comverse after the acquisition of NetCentrex. He now manages Actility, provider of IMS based M2M and smartgrid infrastructure and applications.

A much-needed collection of cutting-edge methods for the detection and discovery of new cancer markers, particularly molecular tumor markers. Each method is fully described by a hands-on expert who regularly uses it in the laboratory and has perfected it to a high degree of ready reproducibility. Special emphasis is given to statistical considerations in the analysis of tumor markers, recent discoveries in the field of serum and tissue marker proteins, cytogenetic markers, molecular markers, and markers for the early detection of cancer. The well-honed procedures detailed here will enable cancer researchers and clinical oncologists alike to realize the promise of these powerful new diagnostic strategies for the detection, therapy, and successful prevention of cancer.

In the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells. In Transcription Factors: Methods and Protocols, experts in the field describe state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function. The chapters are written by prominent scientists, many of whom developed these methods, and highlight protocols that focus on specific transcription factor family members with particular relevance to human disease. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary...

The cell wall and its constituent polysaccharides and proteins control nearly all plant-based biological and biophysical processes. Understanding the cell wall is, therefore, not only fundamental to the plant sciences but is also pertinent to aspects of human and animal nutrition and health as well as plant-microbe and plant-animal interactions. In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism.В The book delves into a range of techniques involving plant tissue culture, which can be applied to investigating cell wall structure and metabolism, methods directed towards structural analysis and occurrence of carbohydrates, the development and use of microscopy-based tools and techniques, procedure...

Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and ...

* An ideal starting point for anyone wanting to learn about next generation wireless networks

* Gives important insights into the design of wireless IP networks

* Illustrates the standards and network architectures defined by leading standards bodies (including MWIF, 3GPP and 3GPP2)

* Discusses protocols in four key areas: signaling, mobility, quality of service, and security

* The authors have a good deal of experience in this field, and have many patents pending in the area of wireless networking